Links to pubmed are also available for selected references. Screening by hep 2 cells is a critical first step in the diagnosis of patients with sard. Elfemf on hep2 cells were carried out using four different conditions. Hep2 cells were treated with chx and infected with vcpc0, which is a virus derived from the wildtype hsv1 strain 17 that generates both copies of icp0 using the viral promotor and cdna copies of. Rsv grown in vero african green monkey cells has a truncated attachment g glycoprotein as compared to that grown in hep2 cells. It is also recommended that healthcare workers be vaccinated. Abstract strong illumination variation is a key challenge in the human epithelial type 2 hep 2. All of the hep 2000 cells are transfected with the information to hyperexpress the 60kd ssaro antigen. The percentages of cells in monolayers, which bound the above antibodies, were also determined by fluorescence microscopy. Exploring illumination robust descriptors for human epithelial type 2 cell classification xianbiao qi, guoying zhao, jie chen, matti pietikainen. They usually produce a peripheral or homogeneous pattern on hep2 cell line. The icpr2012 dataset contains 28 hep2 images with different number of cells in each image.
Finally, remarks of the hep2 cell pattern recognition are presented in section vi. The term antinuclear antibody ana describes a variety of autoantibodies that react with constituents of cell nuclei including dna, proteins and ribonucleoproteins 1,2. They also allow identification of antiro antibodies, because acetone is used for fixation of the cells other fixatives can wash the antigen away. The classification of hep2 cell patterns using fractal descriptor. Origin of hep2 cells used for culture of chlamydiae. The first dose is recommended within 24 hours of birth with either two or three more doses given after that. Discovering discriminative cell attributes for hep2. Following the recent hep2 cells classification contest held at icpr 2012, we extend the scope of research in this field to develop a method of feature comparison that goes beyond the analysis of individual cells and majorityvote decisions to consider the full distribution of cell parameters within a patient sample. Analyzing features by swlda for the classification of hep. The most frequent staining patterns of hep2 cells in clinical practice are as follows 11. Typical patterns of ana using hep2 cells atlas of antinuclear. Sources of variability include preparation of slides, conjugate specificity, and efficiency of the fluorescent microscope and experience of the reader. Add appropriate aliquots of the cell suspension to new culture vessels. Salmonella typhimurium invasion of hep2 epithelial cells.
Pdf antinuclear antibody screening using hep2 cells. Hep2 cell image classification is an important and relatively unexplored area of research. Hepg2 is an immortalized cell line consisting of human liver carcinoma cells, derived from the liver tissue of a 15yearold caucasian male who had a welldifferentiated hepatocellular carcinoma, which is the fifth mostcommon cancer worldwide. Due to the subjective nature of ana screening, the quality of hep2.
The hep2 cell, a native protein array with hundreds of antigens. Hep2 cell image classi cation is an important and relatively unexplored area of research. Vaccine excipient summary excipients included in u. Pdf hep2 cell classification using binary decision tree approach. A research on automation diagonsis of pattern recognition in. Antinuclear antibody screening using hep2 cells protocol. Objectivethe objective of this study was to determine whether. Controversy concerning the antinuclear antibody anapositive rates in patients with systemic lupus erythematous sle has persisted, and many efforts in improving the test quality have been made. Frontiers report of the first international consensus on. Pdf download atlas of antinuclear antibodies mbl medical. Hep2 atcc ccl23 homo sapiens hela contaminant carcinoma.
Palaniappan 1 1 department of computer science, university of missouricolumbia, columbia, mo 65211 usa 2 department of computer science, montclair state university, montclair, nj 07043 usa abstract indirect immunouorescence iif imaging is a method used for detection. Hep2 cell classification in iif images using shareboost. For physical therapists, occupational therapists, athletic trainers, and other qualified rehab professionals. Hep2 cell line shellvial presentation24 shellvials. Autoantibody detection using indirect immunofluorescence. Mbl,diagnostic reagents,atlas of antinuclear antibodies.
Hep g2 is an immortal cell line which was derived from the liver tissue of a 15yearold african american adolescent boy with a welldifferentiated hepatocellular carcinoma. Home new horizons in translational medicine, volume 2, issue 2, 2015 antitumor effects of the human monoclonal antinuclear antibody on the hep2 cells. Antitumor effects of the human monoclonal antinuclear. Icpr hep2 cell classification contest dataset and the new. As a result, when antissaro antibodies are the only anas present, one of. Cell culture cell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells e. Galangin induces autophagy via deacetylation of lc3 by.
In addition to weakened or killed disease antigens viruses or bacteria, vaccines contain very. The hep2 cell, a native protein array with hundreds of antigens, provides an ideal substrate for the detection of ana 1. Recently, there has been a growing interest in developing computer aided diagnostic cad systems for improving the reliability and consistency of pathology test results. Screening by hep2 cells is a critical first step in the diagnosis of patients with sard. In addition to weakened or killed disease antigens viruses or bacteria, vaccines contain very small amounts of other.
Hep 2 cell image classification with deep convolutional neural networks conference paper pdf available in ieee journal of biomedical and health informatics 21 2 august 2014 with 625 reads. We propose a hep2 cell classification framework for mitotic vs nonmitotic cells for detection of autoimmune disorders. The detection of autoantibodies is an important element in the diagnosis and monitoring of disease progression in patients with autoimmune diseases. Human epithelial type 2 hep2 cells, considered to originate from a human laryngeal carcinoma, allow recognition of over 30 different nuclear and cytoplasmic patterns that are given by upwards of 50 different autoantibodies are associated with various autoimmune conditions. Taxonomy of hep2 cell staining patterns used in the cantor project. The detection of antinuclear antibodies, the ana test, is a clear laboratory diagnostic indicator of rheumatic autoimmune disease. V brazilian consensus guidelines for detection of anticell. The detection of ana in human serum is an important screening tool for connective. Speckled hep2 cells, immunofluorescence 16099409590. This paper describes a novel cad system for the antinuclear antibody ana test via indirect immunofluorescence protocol on human epithelial type 2 hep2 cells. The indirect immunofluorescence assay iifa on hep2 cells is widely used for detection of antinuclear antibodies ana. Classification of human epithelial type 2 cell indirect.
Media in category hep 2 cells the following 4 files are in this category, out of 4 total. Galangin suppresses proliferation and induces apoptosis and autophagy in hepatocellular carcinoma hcc cells, but the precise mechanism is not clear. Limitations of antinuclear antibody tests hep2 are. The important aspects of such classification framework is to use convolutional neural network cnn as learned feature extractor, as to incorporate the prominent and distinctive shape and texture differences within the cells. A comparative analysis praful agrawal, mayank vatsa, richa singh indraprastha institute of information technology, delhi, india abstract. C, the hep2 cells on the fat cellembedded gel do not invade the gel, although they show thicker stratification than those on the collagen gel without stromal component cells. The hepg2 cell line is commonly used in drug metabolism and hepatoxicity studies. Until today, antibody detection on hep2 cells is maintaining its central role. There are many nuclear staining patterns seen on hep2 cells. Ana hep2 cells image classification using number, size, shape and localization of targeted cell regions. Sources of variability include preparation of slides, conjugate specificity, and efficiency of the fluorescent microscope and experience of. Automated classification for hep2 cells based on linear local.
Your browser will take you to a web page url associated with that doi name. Hep2 is a cell line derived from hela they were are contamination in an originally hepatic cell line see atcc for details and so are a cervix carcinoma cell line, most useful, because they have a rather big nucleus. Following the recent hep2 cells classification contest held at icpr 2012, we extend the scope of. If you need more information about the product, please fill in the form. The occurrence of antinuclear antibodies anas in patient serum has significant. Clinical phenotypes of patients with antidfs70ledgf antibodies in a routine ana referral cohort. Mitotic vs nonmitotic hep2 cells classification for cad. Clinical relevance of hep2 indirect immunofluorescent patterns. The clinical significance of the dense fine speckled immunofluorescence pattern on hep2 cells for the diagnosis of systemic autoimmune diseases. To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Hep2go online home exercise program rehab physical. Hep2 cell classication in iif images using shareboost i. These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice.
The v brazilian consensus for determination of autoantibodies against cellular constituents on hep2 cells, held in brasilia df, brazil on. In laboratory diagnostic tests for connective tissue and autoimmune liver diseases, indirect immunofluorescence on hep2 cells plays a central role in a multistage diagnostic process. Hep 2 cell image classification is an important and relatively unexplored area of research. Antinuclear antibody screening using hep2 cells carol buchner 1, cassandra bryant 2, anna eslami 3, gabriella lakos 4 1 ifa development manager, inova diagnostics, inc. In total, 1455 cells are extracted from 28 images 721 as train and 734 as test. B, the hep2 cells on the fibroblastembedded gel with air exposure invade the gel more deeply and show thicker stratification than cells without air exposure. However, only about 10% 20% of the cells demonstrate this hyperexpression. Vast majority of these patterns will appear on this site. Infection of hep2 cells with both wildtype strains of hsv1, 17 and kos, in the presence of chx were used as positive controls. Abstractrealtime tracking of virus invasion is crucial for understanding viral infection mechanism, which. Antitumor effects of the human monoclonal antinuclear antibody on the hep2 cells.
In vitro evaluation of electroporated gold nanoparticles. Pdf human epithelial type2 hep2 cells have been accepted as. Infection with respiratory syncytial virus enhances. Effects of stromal fibroblasts and fat cells and an. The public database can be downloaded, after registration, from the download. Hepatitis b vaccine is a vaccine that prevents hepatitis b. When the adherence pattern was not defined after 3 h of incubation, the test was repeated with a 6h incubation period. This article is from international journal of nanomedicine, volume 9. Analysis of dfs70 pattern and impact on ana screening using a. An automatic hep2 specimen analysis system based on. Human respiratory syncytial virus memphis 37 grown in hep.
Invasion of ureaplasma diversum in hep2 cells core reader. During the 12th international workshop on autoantibodies and autoimmunity held in sao paulo, brazil, on august 28, 2014, a full day session was devoted to establishing a consensus on the nomenclature of staining patterns observed in the antinuclear antibody ana indirect immunofluorescence test on hep2 cells. In cases with suspected autoimmune disease, the hep2 test is the recommended screening method, which allows for costeffective and highquality serological diagnosis of various autoimmune diseases. Article information, pdf download for distinctive features of positive anticell antibody. This paper presents an experimental analysis of five different categories of feature sets with four. Six classes of stainedcells images defined in the course of icpr 2012 ana hep2 cells classification contest and their. We hypothesized that the virus grown in hep2 cells would cause more severe clinical symptoms and cause more severe pathology. Frontiers comparison of hep2 and vero cell responses. Hep2go is an online home exercise program tool for rehabilitation professionals. Cells that are difficult to detach may be placed at 37c to. As a result, when antissaro antibodies are the only anas present, one of two distinct patterns may be seen. This cytoplasmic pattern is negative for ribosomal pprotein by elisa but cross reacts with cerebellar antigen.
Glass coverslips with monolayers of hep2 cells or hep2 cells 24 h post infection with rsv at an moi of 1. Patterns of ana observed on hep2 cells by iif provide the clinicians with insight into specificity of autoantibodies present, indications of disease. This includes those with poor immune function such as from hivaids and those born premature. Get a printable copy pdf file of the complete article 403k, or click on a page image below to browse page by page. Prevalence of hep2 celladherent escherichia coli and. This paper presents an experimental analysis of five different categories of feature sets with four different classifiers to determine the best. Distinctive features of positive anticell antibody tests indirect. The images are obtained through a fluorescence microscope 40fold magnification lens coupled with a 50 w mercury vapor lamp. Photothermolysis mediated by gold nanorods modified with. Antissdna is not a useful test in the differential diagnosis of sle tran and pisetsky, 2006. Abstractgold nanorods aunrs have been used in plasmonic photothermal therapy pptt.
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